Nā huahana

1. Nā Kiʻi: Keʻokeʻo a melemele iki paha, ka pauka amorphous.

2. Ke Kumu Hoʻoiho: Porcine gastric mucosa.

3. Ke Kaʻina Hana: Hoʻokaʻawale ʻia ʻo Pepsin mai ka mucosa ʻōpū o ka puaʻa me ka hoʻohana ʻana i kahi ʻenehana unuhi kūʻokoʻa.

4. Nā hōʻailona a me ka hoʻohana ʻana: Hoʻohana nui ia no ka dyspepsia ma muli o ka lawe ʻana i nā meaʻai protein, digestive hypofunction i ka wā hoʻihoʻi a me ka nele o ka proteinase o ka ʻōpū i hoʻokumu ʻia e ka gastritis atrophic maʻi, ka maʻi ʻōpū a me ka anemia malignant. ʻO Pepsin kahi enzyme i hūnā ʻia. i loko o ka digestive track o nā mammals.Hoʻohana ia e wāwahi i nā protein i loko o nā peptides liʻiliʻi i hiki ke komo koke ʻia e ka ʻōpū liʻiliʻi.

5. Nā Hana Biochem/physiol: ʻAʻole e like me nā peptidases ʻē aʻe, pepsin hydrolyzes wale nā ​​mea paʻa peptide, ʻaʻole amide a i ʻole nā ​​loulou ester.ʻO ka kikoʻī cleavage e pili ana i nā peptides me kahi ʻakika ʻala ma kēlā ʻaoʻao kēia ʻaoʻao o ka paʻa peptide, ʻoi aku ka nui inā he mea ʻala a i ʻole ka dicarboxylic amino acid ke koena.Hoʻonui ʻia ka maʻalahi i ka hydrolysis inā loaʻa kahi amino acid i loko o ka sulfur kokoke i ka paʻa peptide, aia kahi amino acid aromatic.ʻOi aku ka maikaʻi e ʻoki ʻo Pepsin ma ka ʻaoʻao carboxyl o ka phenylalanine a me ka leucine, a ma ka ʻaoʻao liʻiliʻi ma ka ʻaoʻao carboxyl o nā koena glutamic acid.ʻAʻole ia e pili i ka valine, alanine, a i ʻole nā ​​loulou glycine.Hiki ke hoʻohana ʻia ʻo ZL-tyrosyl-L-phenylalanine, ZL-glutamyl-L-tyrosine, a i ʻole ZL-methionyl-L-tyrosine ma ke ʻano he substrate no ka ʻeli ʻana o ka pepsin.Kāohi ʻia ka Pepsin e kekahi mau peptides me ka phenylalanine.