Nā huahana

1. Nā Kiʻi: Keʻokeʻo a i ʻole melemele liʻiliʻi, crystalline a i ʻole ka pauka amorphous.

2. Mea Hoʻoemi: Porcine gastric mucosa.

3. Ke Kaʻina Hana: Hoʻokaʻawale ʻia ʻo Pepsin mai ka mucosa ʻōpū o ka puaʻa me ka hoʻohana ʻana i kahi ʻenehana unuhi kūʻokoʻa.

4. Nā hōʻailona a me ka hoʻohana ʻana: Hoʻohana nui ʻia ia no ka dyspepsia ma muli o ka lawe ʻana i nā meaʻai protein, digestive hypofunction i ka wā hoʻōla a me ka nele o ka proteinase o ka ʻōpū i hoʻokumu ʻia e ka maʻi atrophic gastritis, ka maʻi maʻi ʻōpū a me ka anemia malignant. ʻO Pepsin kahi enzyme i hūnā ʻia. i loko o ka digestive track o nā mammals.Hoʻohana ia e wāwahi i nā protein i loko o nā peptides liʻiliʻi i hiki ke komo koke ʻia e ka ʻōpū liʻiliʻi.

5. Nā Hana Biochem/physiol: ʻAʻole e like me nā peptidases ʻē aʻe he nui, pepsin hydrolyzes wale nā ​​mea paʻa peptide, ʻaʻole amide a i ʻole ester linkages.ʻO ka kikoʻī o ka cleavage e pili ana i nā peptides me kahi ʻakika ʻala ma kēlā ʻaoʻao kēia ʻaoʻao o ka paʻa peptide, ʻoi aku ka nui inā he mea ʻala a i ʻole ka dicarboxylic amino acid ke koena.Hoʻonui ʻia ka maʻalahi i ka hydrolysis inā loaʻa kahi sulfur-containing amino acid kokoke i ka paʻa peptide, nona ka amino acid aromatic.ʻOi aku ka maikaʻi o ka ʻoki ʻana o Pepsin ma ka ʻaoʻao carboxyl o ka phenylalanine a me ka leucine, a ma kahi liʻiliʻi ma ka ʻaoʻao carboxyl o nā koena glutamic acid.ʻAʻole ia e pili i ka valine, alanine, a i ʻole nā ​​loulou glycine.Hiki ke hoʻohana ʻia ʻo ZL-tyrosyl-L-phenylalanine, ZL-glutamyl-L-tyrosine, a i ʻole ZL-methionyl-L-tyrosine ma ke ʻano he substrate no ka ʻeli ʻana o ka pepsin.Kāohi ʻia ka Pepsin e kekahi mau peptides me ka phenylalanine.